Journal: Cell Death & Disease

Article Title: CXCL12 loaded-dermal filler captures CXCR4 expressing melanoma circulating tumor cells

doi: 10.1038/s41419-019-1796-6

Figure Lengend Snippet: A Cells recovered from EG and CLG gel were seeded on plates immediately after collection. Microphotographs from EG (panel A) and CLG (panel B) at 3 and 7days (Axiovert10 Microscopy-Zeiss). Scale bar is 20 µm. CLG cells express B c-Kit, C Melan A, S100 and D CXCR4 while EG cells express B α-SMA but do not express c-Kit, Melan A and S100. Cells (1 x 10 4 ) were seeded on glass coverslips, fixed with 4% paraformaldehyde (15 min, 4 °C) and stained with rabbit anti-c-Kit primary antibody, mouse anti-αSMA primary antibody, mouse anti-S100 primary antibody mouse, mouse anti-Melan A primary antibody and mouse anti-human CXCR4 primary antibody (clone 12G5 R&D). DAPI was used to stain the cell nucleus (Carlo Zeiss, Axio Scope.A1). CXCR4 expression was compared to PES43, human melanoma cell line-CXCR4 expressing, FB-1, human anaplastic thyroid cancer cell line- low CXCR4 expressing and murine B16-hCXCR4-GFP cells evaluated in the in vitro/in vivo assay. DAPI was used to stain the cell nucleae. Magnification 400X. Scale bar is 25 µm

Article Snippet: Cells (1 x 10 4 ) were seeded on glass coverslips, fixed with 4% paraformaldehyde (15 min, 4 °C) and stained with rabbit anti-c-Kit primary antibody, mouse anti-αSMA primary antibody, mouse anti-S100 primary antibody mouse, mouse anti-Melan A primary antibody and mouse anti-human CXCR4 primary antibody (clone 12G5 R&D).

Techniques: Microscopy, Staining, Expressing, In Vitro, In Vivo

Journal: Clinical & experimental metastasis

Article Title: Expression of natural killer cell regulatory microRNA by uveal melanoma cancer stem cells

doi: 10.1007/s10585-016-9815-9

Figure Lengend Snippet: (A and B) Examples of blood nucleated cells from patients with metastatic uveal melanoma that were positive for CD271 and negative for CD45 (arrows) and (C) virtual confocal imaged slice cells of cells positive for CD271 and Melan-A (arrow). Nuclei are visualized with DAPI (blue color). (D) Example of the formation of non-adherent spheres by CD271+ circulating cells from a patient with metastatic uveal melanoma cultured for 4 days in stem-cell supportive media.

Article Snippet: Immunofluorescence staining was performed with the following antibodies: anti-CD271 (rabbit polyclonal; Abeam, San Francisco, CA), anti-CD45 pre-conjugated to Alexa 488 (Molecular Probes, Thermo Fisher Scientific) to identify leukocytes, and anti-Melan-A (rabbit monoclonal, Novus Biologicals, Littleton, CO; or mouse clone A103, Dako, Carpinteria, CA).

Techniques: Cell Culture